Crystal structure of the catalytic [alpha] subunit of E. coli replicative DNA Polymerase lll

Article Abstract:

The independent growth of the DNA replication machinery in bacteria is suggested by the significant difference in structure between Polymerase lll (Pol III) and the members of the canonical DNA polymerase families that include eukaroyotic replicative polymerase. The likelihood that all DNA polymerases share a common ancestor is suggested by a structural element near the active site in Pol lll, also highlighting a model for interaction of Pol lll with the sliding clamp and DNA.ddna replicatiopn

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Structure of the C-terminal region of p21WAF1/CIP1 complexed with human PCNA

Article Abstract:

The binding functionality of the human proliferating cell nuclear antigen (PCNA) with p21WAF1/CIP1 complex is located on the C-terminal 22 residue which blocks PCNA-mediated gene replication. The PCNA-p21 complex exhibits a trigonal crystal structure in which one 22 residue peptide is bonded with three PCNA subunits. The complex is composed of 57% peptide and 43% PCNA that form a ring with an uncovered hole in its center.

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Crystal structure of the processivity clamp loader gamma complex of E. coli DNA polymerase III

Article Abstract:

Crystallographic analysis of the clamp loader gamma complex of Escherichia coli DNA polymerase III shows a pentameric arrangement of subunits with their C-terminal domains forming a circular collar and the N-terminal domains binding ATP. The structure indicates that the gamma complex switches between a closed state and an open form.

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